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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
22/12/2020 |
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Data da última atualização: |
28/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARTINS, K. R.; GARCIA, M. V.; BONATTE JUNIOR, P.; DUARTE, P. O.; HIGA, L. O. S. de; CSORDAS, B. G.; BARROS, J. C.; ANDREOTTI, R. |
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Afiliação: |
Kauê Rodriguez Martins; Marcos Valério Garcia; Paulino Bonatte?Junior; Pãmella Oliveira Duarte; Leandro Oliveira Souza de Higa; Bárbara Guimarães Csordas; JACQUELINE CAVALCANTE BARROS, CNPGC; RENATO ANDREOTTI E SILVA, CNPGC. |
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Título: |
Correlation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Experimental and Applied Acarology, jun. 2020. |
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Idioma: |
Inglês |
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Conteúdo: |
The tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation. MenosThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable ... Mostrar Tudo |
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Palavras-Chave: |
IELISA; QPCR; Tick. |
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Thesagro: |
Cerrado. |
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Thesaurus Nal: |
Anaplasmosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219597/1/16-Correlation-between.pdf
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Marc: |
LEADER 02269naa a2200265 a 4500
001 2128625
005 2020-12-28
008 2020 bl uuuu u00u1 u #d
100 1 $aMARTINS, K. R.
245 $aCorrelation between Rhipicephalus microplus ticks and Anaplasma marginale infection in various cattle breeds in Brazil.$h[electronic resource]
260 $c2020
520 $aThe tick Rhipicephalus microplus is responsible for the transmission of Anaplasma marginale, which causes hemolytic anemia, abortion, decreased production, and mortality in cattle in Brazil. However, A. marginale can also persist in cattle herds without any clinical signs. This study investigated the relationship between the number of ticks present on each cattle and the circulating number of A. marginale msp1β gene copies in the blood of Brangus and Nellore cattle reared in the Brazilian Cerrado through a year period. Twenty-three animals (11 Brangus and 12 Nellore) were raised for 12 months with ticks counted every 18 days, and blood collected every 36 days. Blood sera was used for total antigen iELISA, genomic DNA was extracted from whole blood by the phenol/chloroform method and then analyzed by PCR to confirm A. marginale presence with the msp5 gene. Positive samples were quantified by qPCR using msp1β gene. Brangus cattle presented 4.5 fold more ticks than Nellore group. Although Brangus cattle carried a higher overall A. marginale msp1β gene presence than Nellore cattle, no relationship of tick count and copy number could be achieved due to high variability in copy number. Moreover, both breeds showed similar weight gain and a similar serological pattern throughout the year. None of the animals showed any clinical signs of anaplasmosis during the experimental period, indicating that a low level of tick infestation may be sufficient to maintain a stable enzootic situation.
650 $aAnaplasmosis
650 $aCerrado
653 $aIELISA
653 $aQPCR
653 $aTick
700 1 $aGARCIA, M. V.
700 1 $aBONATTE JUNIOR, P.
700 1 $aDUARTE, P. O.
700 1 $aHIGA, L. O. S. de
700 1 $aCSORDAS, B. G.
700 1 $aBARROS, J. C.
700 1 $aANDREOTTI, R.
773 $tExperimental and Applied Acarology, jun. 2020.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
11/02/2016 |
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Data da última atualização: |
11/02/2016 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
CARVALHO, R. N.; BASSINELLO, P. Z.; KOAKUZU, S. N.; ARAÚJO, E. J. de; TEIXEIRA, M. C. |
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Afiliação: |
ROSANGELA NUNES CARVALHO, CNPAF; PRISCILA ZACZUK BASSINELLO, CNPAF; SELMA NAKAMOTO KOAKUZU, CNPAF; EDMAR JOSE DE ARAUJO, CNPAF; MAURO CESAR TEIXEIRA, CNPAF. |
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Título: |
Procedimento de determinação da dureza e pegajosidade de arroz polido cozido em texturômetro. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2015. |
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Páginas: |
4 p. |
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Série: |
(Embrapa Arroz e Feijão. Comunicado técnico, 227). |
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ISSN: |
1678-961X |
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Idioma: |
Português |
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Conteúdo: |
O objetivo é ajustar e estabelecer um procedimento laboratorial para determinação em texturômetro dos parâmetros de dureza e pegajosidade de amostras de arroz branco cozido. |
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Palavras-Chave: |
Dureza de arroz branco; Pegajosidade de arroz branco; Teste sensorial; Texturômetro. |
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Thesagro: |
Arroz polido; Cocção; Propriedade organoleptica; Textura. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138723/1/Com-Tec-227.pdf
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Marc: |
LEADER 01070nam a2200289 a 4500
001 2036590
005 2016-02-11
008 2015 bl uuuu u0uu1 u #d
022 $a1678-961X
100 1 $aCARVALHO, R. N.
245 $aProcedimento de determinação da dureza e pegajosidade de arroz polido cozido em texturômetro.$h[electronic resource]
260 $aSanto Antônio de Goiás: Embrapa Arroz e Feijão$c2015
300 $a4 p.
490 $a(Embrapa Arroz e Feijão. Comunicado técnico, 227).
520 $aO objetivo é ajustar e estabelecer um procedimento laboratorial para determinação em texturômetro dos parâmetros de dureza e pegajosidade de amostras de arroz branco cozido.
650 $aArroz polido
650 $aCocção
650 $aPropriedade organoleptica
650 $aTextura
653 $aDureza de arroz branco
653 $aPegajosidade de arroz branco
653 $aTeste sensorial
653 $aTexturômetro
700 1 $aBASSINELLO, P. Z.
700 1 $aKOAKUZU, S. N.
700 1 $aARAÚJO, E. J. de
700 1 $aTEIXEIRA, M. C.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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